![tbs discovery pro manual tbs discovery pro manual](https://i.ytimg.com/vi/u_Jus7lro6A/maxresdefault.jpg)
M元20 scaffold also holds the promise to deliver additional compounds with further improved biochemical, cellular, and pharmacokinetic properties suitable for investigating in vivo roles of GSK3b in pertinent animal physiology and pathology. Taken together, M元20 is a potent and highly selective small molecular probe against GSK3b, allowing better investigation and interpretation of GSK3b cellular functions than the existing prior art. At the same time, no cellular toxicity by M元20 was observed in SH-SY5Y cells at the highest testing concentration of 30 μM. Further, M元20 demonstrates excellent cellular activity in inhibiting GSK3b-mediated Tau phosphorylation in SH-SY5Y neuroblastoma cells (IC 50 of 1 μM), and in relieving negative regulation by GSK3b on cellular beta-catenin degradation and TCF/LEF promoter activities with EC 50 of 5 μM in both assays. M元20 has a superior kinome-wide selectivity profile compared to CHIR99021.
![tbs discovery pro manual tbs discovery pro manual](https://deanfourie.me/wp-content/uploads/2018/06/10922727_337827346421084_3187456470673923559_n-920x690.jpg)
Subsequent chemical modifications of CID 5706819, guided by a co-crystal structure with GSK3b, and a battery of biochemical and cell-based assays led to CID 56840716/ M元20 that inhibits GSK3b at IC 50 between 10–30 nM. Among the inhibitors identified, CID 5706819 showed decent potency and excellent selectivity inhibiting only five out of over three hundred kinases at 10 μM by over fifty percent.
![tbs discovery pro manual tbs discovery pro manual](https://cdn.3dforprint.com/modelos/4024/tbs-discovery-pro-gimbal-platfom3.jpg)
A library of over three hundred twenty thousand compounds was screened against human GSK3b. The goal for this project is to identify potent and highly selective small molecule probes to investigate GSK3b biology in cellular and ultimately in whole animal models. Many inhibitors of GSK3b exist, however these compounds have been found lacking in selectivity, with CHIR 99021 considered most potent and selective. Precisely how GSK3b maintains its pathway specificity efficiently at the crossroads of many cellular processes is still unclear. Consequently, this enzyme has been implicated in multiple human disorders including Alzheimer’s disease, bipolar disorder, noninsulin-dependent diabetes mellitus, cardiac hypertrophy, and cancer. I can't give you any advice on Futaba/Pixhawk I'm afraid though.The serine/threonine kinase glycogen synthase kinase-3 beta (GSK3b) is a known master regulator for several cellular pathways that include insulin signaling and glycogen synthesis, neurotrophic factor signaling, Wnt signaling, neurotransmitter signaling and microtubule dynamics. A nice thing is that you can mix and match with the ordinary outputs, so for example, I had the regular outputs going straight to the WKM which sat right next to it and then the SBUS with decoders for the more physically distant stuff like the pilot cam's pan-tilt controls. I'm using a UHF system on my 14SG with all my birds now, but can tell you that the SBUS was really nice to use on my S800, once I figured it out. I am looking forward to learning all their is to know about this controller and using/applying all its great features! any one have experience with Pixhawk/Futaba SBus? I did order the PPM encoder board, so if push comes to shove, I may set it up with that although I would like to learn the Sbus setup and use it (my understanding is that the SBUS is besides being "one wire" it is a "faster" connection as well). I have been reading about setting the Pixhawk up with Sbus and it seems that there are still issues with the 7008 receiver that came with the 14SG. My initial plan was set it up with my DX8, but now plan to fly it with a Futaba 14SG. I have been reading reading reading all I can find about the Pixhawk. Just finishing a build of an Extreme Spyder Spyder Extreme FPV Hexacopter - YouTube